Identification of phase II metabolites of thiol-conjugated [6]-shogaol in mouse urine using high-performance liquid chromatography tandem mass spectrometry
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چکیده
منابع مشابه
High-performance liquid chromatography–tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites
Applications of tandem mass spectrometry (MS/MS) techniques coupled with high-performance liquid chromatography (HPLC) in the identification and determination of phase I and phase II drug metabolites are reviewed with an emphasis on recent papers published predominantly within the last 6 years (2002-2007) reporting the employment of atmospheric pressure ionization techniques as the most promisi...
متن کاملUsefulness of Derivatization in High-Performance Liquid Chromatography/Tandem Mass Spectrometry of Conjugated Vitamin D Metabolites
larly the oxidation of its side chain, has been investigated by many groups, the conjugates of D metabolites still remain poorly understood. Axelson1 and we2 have reported that 25-hydroxyvitamin D3 [25(OH)D3] 3-sulfate (S) is a major circulating metabolite in humans. Furthermore, we identified the monoglucuronides (G) of D compounds {vitamin D2, vitamin D3 (D3), 25hydroxyvitamin D2, 25(OH)D3, a...
متن کاملIdentification of finasteride metabolites in human bile and urine by high-performance liquid chromatography/tandem mass spectrometry.
The objective of this study was to further investigate the metabolism of the 5alpha-reductase inhibitor, finasteride, and to identify previously unknown phase I and phase II metabolites in vitro and in vivo in human bile and urine. Healthy volunteers were given 5 mg of finasteride, directly to the intestine, and bile and urine were collected for 3 and 24 h, respectively. A single-pass perfusion...
متن کاملUsing liquid chromatography-tandem mass spectrometry to quantify monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine.
We present an assay which employs enzyme digestion and solid phase extraction followed by liquid chromatography-tandem mass spectrometry to simultaneously quantify 16 hydroxylated polycyclic aromatic hydrocarbons (OHPAHs) in 3-ml samples of urine. The analytes consisted of 2-, 3-, and 4-ring OHPAHs, namely, 1- and 2-hydroxynaphthalene (1- and 2-OHNAP), 2-hydroxyfluorine (2-OHFLU), 1-, 2-, 3-, 4...
متن کاملMeasurement of caffeine and five of the major metabolites in urine by high-performance liquid chromatography/tandem mass spectrometry.
Analysis of caffeine and its metabolites is of interest with respect to caffeine exposure, for kinetic and metabolism studies and for opportunistic in vivo estimation of drug metabolizing enzyme activity in humans and animals. For the latter, analysis is usually done by high-performance liquid chromatography (HPLC) with UV detection. However, this method is close to the detection limit for cert...
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ژورنال
عنوان ژورنال: Journal of Chromatography B
سال: 2012
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2012.09.020